Nanotips for single-step preparation of DNA for qPCR analysis.

نویسندگان

  • Dinesh Kalyanasundaram
  • Jong-Hoon Kim
  • Gareth Fotouhi
  • Hyun-Boo Lee
  • Morgan Hiraiwa
  • Kieseok Oh
  • Kyong-Hoon Lee
  • Jae-Hyun Chung
چکیده

A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.

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عنوان ژورنال:
  • The Analyst

دوره 138 11  شماره 

صفحات  -

تاریخ انتشار 2013